An attempt of biocontrol the tomato-wilt disease caused by Verticillium dahliae using Burkholderia gladioli pv.\ud agaricicola and its bioactive secondary metabolites

摘要

new microbial natural biocides such as\udmicrobial secondary metabolites to reduce\udthe environmental pollution due to the\udexcessive use of synthetic pesticides.\udVerticillium wilt, caused by the soil-borne\udVerticillium dahliae, is a widespread disease\udin tomato growing in many parts of the\udworld. Burkholderia gladioli pv. agaricicola\udproduces some antimicrobial substances\udand extracellular hydrolytic enzymes which\udexhibited promising antimicrobial activity\udtowards several phytopathogens. The aims\udof the current research are to assess in vitro\udfungicidal effect of 4 strains of B. gladioli\udpv. agaricicola (ICMP11096, 11097, 12220\udand 12322) against V. dahliae using culture\udor cell-free culture filtrate. In situ assay was\udperformed to evaluate the biocontrol effect\udof the most efficient bacterial strain on wilt\uddisease caused by V. dahliae in tomato\udplants. Results demonstrated that the studied\udbacterial strain ICMP12322 exerted the\udhighest in vitro antifungal activity against V.\uddahliae which correlated with its ability to\udproduce extracellular hydrolytic enzymes.\udFurthermore, in situ results showed that the\udselected bacterial strain significantly minimized\udthe disease incidence.